Mouse Monoclonal Antibody to 4E-BP1/2/3 (Phospho-Thr 45)

包装: 1G|100G|500G|1KG|100KG

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别名:4E-BP1 antibody 4EBP1 antibody 4EBP1_HUMAN antibody BP 1 antibody eIF4E binding protein 1 antibody eIF4E-binding protein 1 antibody Eif4ebp1 antibody Eukaryotic translation initiation factor 4E-binding protein 1 antibody PHAS-I antibody PHASI antibody Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
应用:WB,IP
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5′ cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.

Description

The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5′ cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.

Specification
Aliases	4E-BP1 antibody 4EBP1 antibody 4EBP1_HUMAN antibody BP 1 antibody eIF4E binding protein 1 antibody eIF4E-binding protein 1 antibody Eif4ebp1 antibody Eukaryotic translation initiation factor 4E-binding protein 1 antibody PHAS-I antibody PHASI antibody Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Entrez GeneID	19;
Swissprot	O95477
WB Predicted band size	21kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	peptide
Formulation	1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Application
IP	1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
WB	1/1000-1/1000

	Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
	Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).