库存-现货
瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to ALDOC

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  • 满200元免运费
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  • 现货闪电 当时发货

别名:Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Specification
Aliases Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDOC, ALDC
Entrez GeneID 230
Swissprot P09972
WB Predicted band size 39.5kDa
Host/Isotype Mouse IgG3
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen Purified His-tagged ALDOC protein was used to produced this monoclonal antibody.
Formulation Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-ALDOC Antibody (C-term) at 1:2000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: 293T/17 whole cell lysate
Lane 3: mouse brain lysate
Lane 4: rat brain lysate
Lane 5: mouse spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 39 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HL-60 cells stained with P33308(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33308, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG3 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。