Mouse Monoclonal Antibody to ATP Citrate Lyase

包装: 1G|100G|500G|1KG|100KG

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别名:ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme
应用:WB,ICC,FCM
反应种属:Human,Mouse,Monkey
规格：50μl/100μl

Description
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.

Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.

Specification
Aliases	ACLY; ATP-citrate synthase; ATP-citrate; pro-S-)-lyase; ACL; Citrate cleavage enzyme
Entrez GeneID	47
Swissprot	P53396
clone	3G9
WB Predicted band size	Calculated MW: 121 kDa; Observed MW: 121 kDa
Host/Isotype	Mouse IgG2a
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Monkey
Immunogen	Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of ATPCitrate Lyase in 3T3, K562, COS7 and Hela lysates using ATPCitrate Lyase antibody.
	Immunocytochemistry analysis of ATP Citrate Lyase in HeLa cells using ATPCitrate Lyase (Cterminus) antibody.
	Flow Cytometry analysis of HeLa cells stained with ATPCitrate Lyase (red). Black line histogram represents the isotype control, normal mouse IgG