Mouse Monoclonal Antibody to CAPN2

包装: 1G|100G|500G|1KG|100KG

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别名:Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Specification
Aliases	Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
Entrez GeneID	824
Swissprot	P17655
WB Predicted band size	80.0kDa
Host/Isotype	Mouse IgG2b
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/100

	Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody(Cat. #P32636). P32636 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
	Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#P32636) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#P32636). P32636 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.