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瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to CD34

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别名:Hematopoietic progenitor cell antigen CD34, CD34, CD34
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.
Specification
Aliases Hematopoietic progenitor cell antigen CD34, CD34, CD34
Entrez GeneID 947
Swissprot P28906
WB Predicted band size 40.7kDa
Host/Isotype Mouse IgG1
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This CD34 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the Central region of human CD34.
Formulation Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/1000

Anti-CD34 Antibody (Center) at 1:1000 dilution + NIH/3T3 whole cell lysate
Secondary
Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 40716 Da

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human appendix tissue using P30487 performed on the Leica? BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(Cat#P30487). P30487 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。