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别名:AOM; ANFH; SEDC; STL1; COL11A3应用:WB,ICC,FCM
反应种属:Human
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | AOM; ANFH; SEDC; STL1; COL11A3 |
| Entrez GeneID | 1280 |
| Swissprot | P02458 |
| clone | 7G2C8 |
| WB Predicted band size | 141.8kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human COL2A1 (AA: 1222-1487) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| ICC | 1/50 – 1/200 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) |
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Western blot analysis using COL2A1 mouse mAb against Hela (1), MCF-7 (2),A549 (3),Jurkat (4), and K562 (5) cell lysate. |
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Immunofluorescence analysis of Hela cells using COL2A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of Jurkat cells using COL2A1 mouse mAb (green) and negative control (red). |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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