中文 
别名:DBX; DDX3; HLP2; DDX14; CAP-Rf应用:WB,IHC,ICC,FCM
反应种属:Human,Mouse,Monkey,Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | DBX; DDX3; HLP2; DDX14; CAP-Rf |
| Entrez GeneID | 1654 |
| Swissprot | O00571 |
| clone | 3B9E3 |
| WB Predicted band size | 73.2kDa |
| Host/Isotype | Mouse IgG2a |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey,Rat |
| Immunogen | Purified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| IHC | 1/200 – 1/1000 |
| ICC | 1/200 – 1/1000 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) |
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Western blot analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate. |
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Immunofluorescence analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. |
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Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red). |
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Flow cytometric analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red). |
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using DDX3X mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX3X mouse mAb with DAB staining. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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