Mouse Monoclonal Antibody to DNA PKcs

包装: 1G|100G|500G|1KG|100KG

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别名:PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
应用:WB,IHC,ICC,IP
反应种属:Human
规格：50μl/100μl

Description
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Description

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Specification
Aliases	PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
Entrez GeneID	5591
Swissprot	P78527
clone	7G3
WB Predicted band size	Calculated MW: 469 kDa; Observed MW: 450 kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of DNAPKcs in Hela and K562 lysates using DNAPKcs antibody.
	Western blot analysis of DNAPKcs in Hela, Molt4, A549 and HCT116 lysates using DNAPKcs antibody.
	Immunoprecipitation analysis of DNA PKcs in Hela lysates using DNAPKcs antibody.
	Immunocytochemistry analysis of DNA PKcs in Hela using DNAPKcs antibody.
	Immunohistochemistry analysis of paraffin-embedded Breast cancer using DNAPKcs antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.