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瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to ENOG

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别名:Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
This gene encodes one of the three enolase isoenzymes
found in mammals. This isoenzyme, a homodimer, is found in mature
neurons and cells of neuronal origin. A switch from alpha enolase
to gamma enolase occurs in neural tissue during development in rats
and primates.
Specification
Aliases Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Entrez GeneID 2026
Swissprot P09104
WB Predicted band size 47.3kDa
Host/Isotype Mouse IgG2b
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ENOG monoclonal antibody is generated from mouse immunized with ENOG recombinant protein.
Formulation Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Application
WB 1/500-1/1000
IHC 1/100-1/2000

All lanes : Anti-ENOG at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: CEM whole cell lysate
Lane 3: U-251 MG whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 47 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human pancreas section using Pink1(Cat#P34656). P34656 was diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
ENOG Antibody (Cat. #P34656)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。