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瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to GLS2

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别名:Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Specification
Aliases Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA
Entrez GeneID 27165
Swissprot Q9UI32
WB Predicted band size 66.3kDa
Host/Isotype Mouse IgG1
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2.
Application
WB 1/1000-1/2000
IHC 1/100-1/500

All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilution
Lane 1: human brain lysate
Lane 2: mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 66 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P34336 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。