别名:Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. |
Specification | |
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Aliases | Glutaminase liver isoform, mitochondrial, GLS, 3.5.1.2, L-glutaminase, L-glutamine amidohydrolase, GLS2, GA |
Entrez GeneID | 27165 |
Swissprot | Q9UI32 |
WB Predicted band size | 66.3kDa |
Host/Isotype | Mouse IgG1 |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This GLS2 antibody is generated from a mouse immunized with a recombinant protein of human GLS2. |
Application | |
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WB | 1/1000-1/2000 |
IHC | 1/100-1/500 |
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All lanes : Anti-GLS2 Antibody at 1:1000-1:2000 dilution Lane 1: human brain lysate Lane 2: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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P34336 staining GLS2 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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