Mouse Monoclonal Antibody to GRP78 BiP

包装: 1G|100G|500G|1KG|100KG

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别名:HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Description

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Specification
Aliases	HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
Entrez GeneID	3309
Swissprot	P11021
clone	7B8
WB Predicted band size	Calculated MW: 72 kDa; Observed MW: 78 kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthetic peptide conjugated to KLH.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of GRP78 BiP in CHO-K1, rat brain, C6, 3T3, Hela lysates using GRP78 BiP antibody
	Western blot analysis of GRP78 BiP in Jurkat, MCF-7, SKBR3, Hela lysates using GRP78 BiP antibody
	Immunoprecipitation analysis of GRP78 BiP in Hela lysates using BiGRP78 BiP antibody.
	Immunocytochemistry analysis of GRP78 BiP in HeLa cells using GRP78 BiP antibody.
	Immunohistochemical analysis of paraffin-embedded Human tonsils using GRP78 BiP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	Immunohistochemistry analysis of paraffin-embedded Human Colon Caricnoma using GRP78 BiP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.