中文 
别名:应用:WB,IP
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Neu (ErbB-2 erythroblastic leukemia viral oncogene homolog 2, HER-2, NGL, TKR1, c-erb B2) oncogene was originally cloned from a rat neuroglioblastoma. Human Neu is referred to as HER-2 since the protein structure resembles human epidermal growth factor receptor (HER). ErbB-2 refers to a high level of similarity to ErbB (avian erythroblastosis oncogene B), later found to code for EGFR (HER). Tyr 1248-phosphorylated Neu localizes with Mucin 4/sialomucin complex at the apical surfaces of ductal and alveolar cells in rodent lactating gland. Phosphorylation of Neu at Tyr 1139 promotes association of GRB2 and GRB7 through an Src homology 2 (SH2) domain-dependent interaction and contributes to the etiology of certain breast, gastric and esophageal cancers and testicular germ cell tumors. Neu phosphorylation on Tyr 1221 and Tyr 1248 promotes association of Shc (SH2 domain-containing transforming protein 1) through an SH2 domain. Neu phosphorylation at Tyr 1196 and Tyr 1248 promotes association of Shc through a PTB (phosphotyrosine binding) domain. SH2 and PTB domains recognize tyrosine phosphorylated proteins in a sequence-specific fashion and transduce extracellular signals via subcellular targeting, directing assembly of complexes and modulating enzymatic activity. |
| Specification | |
|---|---|
| Entrez GeneID | 2064; |
| Swissprot | P04626 |
| WB Predicted band size | 185kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Amino acid residues surrounding Threonine 686 of Neu of human origin. |
| Formulation | PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Application | |
|---|---|
| IP | IP/1-2μg per100-500μg of total protein(1 ml of cell lysate) |
| WB | 1/1000-1/1000 |
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Western blot analysis of Neu phosphorylation in serum starved A431 (A), and serum starved A431 treated for 15 minutes with PMA (B), LPA (C), Ceramide (D), Bradykinin (E) and Bombesin (F) whole cell lysates. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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