Mouse Monoclonal Antibody to HP1 alpha

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别名:CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha
应用:WB,IHC
反应种属:Human,Rat,Mouse
规格：50μl/100μl

Description
Component of heterochromatin that recognizes and binds histone H3 tails methylated at ‘Lys-9’ (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when ‘Tyr-41’ of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Description

Component of heterochromatin that recognizes and binds histone H3 tails methylated at ‘Lys-9’ (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when ‘Tyr-41’ of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Specification
Aliases	CBX5; HP1A; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha
Entrez GeneID	23468
Swissprot	P45973
clone	12F8
WB Predicted band size	Calculated MW: 22 kDa; Observed MW: 22 kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat,Mouse
Immunogen	Purified recombinant protein expressed in E.coli.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of HP1 alpha in COS7, Hela lysates using HP1 alpha antibody.
	Western blot analysis of HP1α in Hela, A549, HL-60, U2OS, C6 lysates using HP1 alpha antibody
	Immunohistochemistry analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP1 alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	Immunohistochemical analysis of paraffin-embedded Human tonsils using HP1 alpha antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	Immunohistochemistry analysis of paraffin-embedded Human Placenta Tissue using HP1 alpha antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.