中文 
别名:CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322; HSPB1应用:WB,IHC,ICC,FCM
反应种属:Human,Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322; HSPB1 |
| Entrez GeneID | 3315 |
| Swissprot | P04792 |
| clone | 5D7 |
| WB Predicted band size | 27kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human HSP27 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| IHC | 1/200 – 1/1000 |
| ICC | 1/200 – 1/1000 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng); |
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Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate. |
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Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. |
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Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple). |
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Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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