别名:RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52应用:WB,IHC,ICC,FCM
反应种属:Human, Monkey
规格:50μl/100μl
| Specification | |
|---|---|
| Aliases | RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52 |
| Entrez GeneID | 3308 |
| Swissprot | P34932 |
| clone | 2E4F10B11 |
| WB Predicted band size | 94.3kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Monkey |
| Immunogen | Purified recombinant fragment of human HSP70 (AA: 642-841) expressed in mammalian. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| IHC | 1/200 – 1/1000 |
| ICC | 1/200 – 1/1000 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) |
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Western blot analysis using HSP70 mouse mAb against Hela (1), HepG2 (2),Hek293 (3),COS-7 (4),A549 (5) and Jurkat (6) cell lysate. |
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Immunofluorescence analysis of Hela cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of Jurkat cells using HSP70 mouse mAb (green) and negative control (red). |
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Flow cytometric analysis of K562 cells using HSP70 mouse mAb (green) and negative control (red). |
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Flow cytometric analysis of Raji cells using HSP70 mouse mAb (green) and negative control (red). |
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using HSP70 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSP70 mouse mAb with DAB staining. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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