Mouse Monoclonal Antibody to HSPA1A

包装: 1G|100G|500G|1KG|100KG

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别名:Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
应用:WB,ICC
反应种属:Human
规格：50μl/100μl

Description
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Description

This intronless gene encodes a 70kDa heat shock protein
which is a member of the heat shock protein 70 family. In
conjuction with other heat shock proteins, this protein stabilizes
existing proteins against aggregation and mediates the folding of
newly translated proteins in the cytosol and in organelles. It is
also involved in the ubiquitin-proteasome pathway through
interaction with the AU-rich element RNA-binding protein 1. The
gene is located in the major histocompatibility complex class III
region, in a cluster with two closely related genes which encode
similar proteins.

Specification
Aliases	Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Swissprot	P08107
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This HSPA1A antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 574-600 amino acids from human HSPA1A.
Formulation	Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Application
WB	1/100-1/2000
ICC	1/10-1/50

	HSPA1A antibody (Cat. #P30987) western blot analysis in HL-60 cell line lysates (35μg/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).
	Confocal immunofluorescent analysis of HSPA1A Antibody (Cat#P30987) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).