中文 
别名:应用:FCM
反应种属:Human
规格：50μl/100μl
| Specification | |
|---|---|
| Swissprot | unknow |
| Host/Isotype | Mouse IgG2a |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Formulation | Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Application | |
|---|---|
| FCM | 1/50 |
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Overlay histogram showing PHA-stimulated huuman peripheral blood lymphocytes stained with CD25 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37ºC. The secondary antibody used was Goat Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1 µg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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