库存-现货
瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to LC3 (APG8)

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别名:Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
应用:WB
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
Specification
Aliases Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Swissprot Q9H492;Q9GZQ8
Host/Isotype Mouse IgG1
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Formulation Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Application
WB 1/1000

All lanes : Anti-MAP1LC3A Antibody at dilution
Lane 1: Mouse brain whole cell lysate
Lane 2: Rat brain whole cell lysate
Lane 3: Human brain whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-LC3 Mab (Cat. #P34799) at 8 μg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
Western blot analysis of anti-LC3 Mab (Cat. #P34799) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。