中文 
别名:Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Specification | |
|---|---|
| Aliases | Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2 |
| Entrez GeneID | 8932 |
| Swissprot | Q9UBB5 |
| WB Predicted band size | 43.3kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MBD2 antibody is generated from a mouse immunized with a recombinant protein between 10-228 amino acids from human MBD2. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
 |
All lanes : Anti-MBD2 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
P34383 staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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