Mouse Monoclonal Antibody to MBP

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein, MBP
应用:WB,ICC
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called ‘Golli-MBP’) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq].

Description

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called ‘Golli-MBP’) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq].

Specification
Aliases	Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein, MBP
Entrez GeneID	4155
Swissprot	P02686
WB Predicted band size	33.1kDa
Host/Isotype	Mouse IgM
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified His-tagged MBP protein(Fragment) was used to produced this monoclonal antibody.
Formulation	Mouse monoclonal antibody supplied in crude ascites with 0.05% sodium azide.

Application
WB	1/1000
ICC	1/10-1/50

	MBP Antibody (Ascites)(Cat. #P31616) western blot analysis in ZR-75-1 cell line lysates (35μg/lane).This demonstrates the MBP antibody detected the MBP protein (arrow).
	Confocal immunofluorescent analysis of MBP Antibody (Ascites)(Cat#P31616) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).DAPI was used to stain the cell nuclear (blue).