中文 
别名:NAD-dependent malic enzyme, mitochondrial, NAD-ME, 1.1.1.38, Malic enzyme 2, ME2应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | NAD-dependent malic enzyme, mitochondrial, NAD-ME, 1.1.1.38, Malic enzyme 2, ME2 |
| Entrez GeneID | 4200 |
| Swissprot | P23368 |
| WB Predicted band size | 65.4kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
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All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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P34382 staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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