中文 
别名:Methylated-DNA–protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT应用:WB,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Specification | |
|---|---|
| Aliases | Methylated-DNA–protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT |
| Entrez GeneID | 4255 |
| Swissprot | P16455 |
| WB Predicted band size | 21.6kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MGMT antibody is generated from a mouse immunized with a recombinant protein. |
| Application | |
|---|---|
| WB | 1/1000-1/2000 |
| FCM | 1/25 |
 |
All lanes : Anti-MGMT Antibody at1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: MOLT-4 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing Jurkat cells stained with P33310 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33310, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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