Mouse Monoclonal Antibody to MS4A1/CD20

包装: 1G|100G|500G|1KG|100KG

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别名:B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.

Description

This gene encodes a member of the membrane-spanning 4A
gene family. Members of this nascent protein family are
characterized by common structural features and similar intron/exon
splice boundaries and display unique expression patterns among
hematopoietic cells and nonlymphoid tissues. This gene encodes a
B-lymphocyte surface molecule which plays a role in the development
and differentiation of B-cells into plasma cells. This family
member is localized to 11q12, among a cluster of family members.
Alternative splicing of this gene results in two transcript
variants which encode the same protein.

Specification
Aliases	B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Entrez GeneID	931
Swissprot	P11836
WB Predicted band size	33.1kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This MS4A1/CD20 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 266-294 amino acids from the C-terminal region of human MS4A1/CD20.
Formulation	Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/500-1/1000
IHC	1/100-1/1000

	MS4A1/CD20 Antibody (C-term) (Cat. #P31691) western blot analysis in Ramos cell line lysates (35μg/lane).This demonstrates the MS4A1/CD20 antibody detected the MS4A1/CD20 protein (arrow).
	P31691 staining MS4A1/CD20 in human tonsil tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/100) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(Cat#P31691). P31691 was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.