中文 
别名:PDHA; PDHAD; PHE1A; PDHCE1A应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | PDHA; PDHAD; PHE1A; PDHCE1A |
| Entrez GeneID | 5160 |
| Swissprot | P08559 |
| clone | 1G10C10 |
| WB Predicted band size | 43 kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| IHC | 1/200 – 1/1000 |
| ICC | 1/50 – 1/200 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng) |
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Western blot analysis using PDHA1 mouse mAb against HepG2 (1), HEK293 (2), HL-60 (3), SK-OV-3 (4), PC-3 (5), PANC-1 (6), NRK (7), C2C12 (8), C6 (9) and PC-12 (10) cell lysate. |
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Immunoprecipitation using PDHA1 mouse mAb(dilution: 1/250) against HL-60, C2C12, and NRK cell lysate. Western blot analysis using PDHA1 mouse mAb, anti-mouse IgG was used as secondary antibody. Lane 1: cell lysate, Lane 2: Normal Mouse IgG instead of PDHA1 mouse mAb IP in cell lysate, Lane 3: PDHA1 mouse mAb IP in cell lysate. |
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Immunofluorescence analysis of Hela cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Immunofluorescence analysis of NIH/3T3 cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red). |
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Flow cytometric analysis of Jurkat cells using PDHA1 mouse mAb (green) and negative control (red). |
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PDHA1 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PDHA1 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using PDHA1 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using PDHA1 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissues using PDHA1 mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded Rabbit kidney tissues using PDHA1 mouse mAb with DAB staining. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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