Mouse Monoclonal Antibody to PRKAA1

包装: 1G|100G|500G|1KG|100KG

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别名:5′-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Description

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Specification
Aliases	5′-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1
Entrez GeneID	5562
Swissprot	Q13131
WB Predicted band size	64.0kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PRKAA1 Monoclonal antibody was raised using purified His-tagged recombinant human PRKAA1.
Formulation	Mouse monoclonal antibody supplied in crude ascites with 0.05% sodium azide.

Application
WB	1/1000-1/5000
IHC	1/100-1/500

	All lanes : Anti-PRKAA1 Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MDA-MB-468 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Anti-PRKAA1 Antibody (Ascites) at 1:8000 dilution + K562 whole cell lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64009 Da Blocking/Dilution buffer: 5% NFDM/TBST.
	Anti-PRKAA1 Antibody (Ascites) at 1:4000 dilution + MDA-MB-453 whole cell lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64009 Da Blocking/Dilution buffer: 5% NFDM/TBST.
	PRKAA1 Monoclonal( Ascites) (Cat. #P34236) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRKAA1 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.