Mouse Monoclonal Antibody to PSMA5

包装: 1G|100G|500G|1KG|100KG

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别名:Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
应用:WB,IHC,ICC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

Description

The proteasome is a multicatalytic proteinase complex with
a highly ordered ring-shaped 20S core structure. The core structure
is composed of 4 rings of 28 non-identical subunits; 2 rings are
composed of 7 alpha subunits and 2 rings are composed of 7 beta
subunits. Proteasomes are distributed throughout eukaryotic cells
at a high concentration and cleave peptides in an
ATP/ubiquitin-dependent process in a non-lysosomal pathway. An
essential function of a modified proteasome, the immunoproteasome,
is the processing of class I MHC peptides. This gene encodes a
member of the peptidase T1A family, that is a 20S core alpha
subunit.

Specification
Aliases	Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
Entrez GeneID	5686
Swissprot	P28066
WB Predicted band size	26.4kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.
Formulation	Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Application
WB	1/500-1/1000
IHC	1/100-1/500
ICC	1/25

	All lanes : Anti-PSMA5 Antibody at 1:1000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
	PSMA5 Antibody (Cat. #P33306) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).
	Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
	Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody(Cat#P33306). P33306 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.