库存-现货
瑞威尔生物科技 REVERE

Mouse Monoclonal Antibody to RAB18

现货 支持定制

  • 满200元免运费
  • 正品保障 提供发票
  • 现货闪电 当时发货

别名:Ras-related protein Rab-18, RAB18
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration.
Specification
Aliases Ras-related protein Rab-18, RAB18
Entrez GeneID 22931
Swissprot Q9NP72
WB Predicted band size 23.0kDa
Host/Isotype Mouse IgG1
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This RAB18 antibody is generated from a mouse immunized with a recombinant protein of human RAB18.
Formulation Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Application
WB 1/500-1/2000
FCM 1/25

All lanes : Anti-RAB18 Antibody at 1:500-1:2000 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: mouse brain lysate
Lane 3: rat brain lysate
Lane 4: human brain lysate
Lane 5: human testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 23 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing PC-3 cells stained with P33518(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33518, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

暂无评论

发表回复

您的邮箱地址不会被公开。 必填项已用 * 标注


本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。