别名:Ras-related protein Rab-18, RAB18应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl
| Description |
|---|
| Plays a role in apical endocytosis/recycling. May be implicated in transport between the plasma membrane and early endosomes. Plays a key role in eye and brain development and neurodegeneration. |
| Specification | |
|---|---|
| Aliases | Ras-related protein Rab-18, RAB18 |
| Entrez GeneID | 22931 |
| Swissprot | Q9NP72 |
| WB Predicted band size | 23.0kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB18 antibody is generated from a mouse immunized with a recombinant protein of human RAB18. |
| Formulation | Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Application | |
|---|---|
| WB | 1/500-1/2000 |
| FCM | 1/25 |
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All lanes : Anti-RAB18 Antibody at 1:500-1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: human brain lysate Lane 5: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing PC-3 cells stained with P33518(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33518, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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