中文 
别名:Ras-related protein Rab-27B, C25KG, RAB27B应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| May be involved in targeting uroplakins to urothelial apical membranes. |
| Specification | |
|---|---|
| Aliases | Ras-related protein Rab-27B, C25KG, RAB27B |
| Entrez GeneID | 5874 |
| Swissprot | O00194 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B. |
| Application | |
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
 |
All lanes : Anti-RAB27B Antibody at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing A431 cells stained with P33516(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33516, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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