Mouse Monoclonal Antibody to Rad23B

包装: 1G|100G|500G|1KG|100KG

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别名:RAD23B; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat,Monkey,Hamster
规格：50μl/100μl

Description
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Description

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Specification
Aliases	RAD23B; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58
Entrez GeneID	5887
Swissprot	P54727
clone	7D11
WB Predicted band size	Calculated MW: 43 kDa; Observed MW: 58 kDa
Host/Isotype	Mouse IgG2b
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Monkey,Hamster
Immunogen	Purified recombinant human hHR23b protein fragments expressed in E.coli.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of hHR23b in A431, K562, Jurkat, C6, 3T3 and Hela lysates using hHR23b antibody.
	Western blot analysis of Rad23B in K562, SW480, CHO-K1, 3T3 and COS7 lysates using Rad23B antibody
	Immunocytochemistry analysis of Rad23B in HeLa using hHR23b antibody.
	Immunohistochemistry analysis of paraffin-embedded Prostate Cancer using hHR23b antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.