别名:Ras association domain-containing protein 2, RASSF2, KIAA0168应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation. |
Specification | |
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Aliases | Ras association domain-containing protein 2, RASSF2, KIAA0168 |
Entrez GeneID | 9770 |
Swissprot | P50749 |
WB Predicted band size | 37.8kDa |
Host/Isotype | Mouse IgG2a |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This RASSF2 antibody is generated from a mouse immunized with a recombinant protein between 1-326 amino acids from human RASSF2. |
Application | |
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WB | 1/2000 |
FCM | 1/25 |
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Anti-RASSF2 Antibody at 1:2000 dilution + Raji whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing Raji cells stained with P33588(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33588, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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