Mouse Monoclonal Antibody to RET

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Description

This gene, a member of the cadherin superfamily, encodes
one of the receptor tyrosine kinases, which are cell-surface
molecules that transduce signals for cell growth and
differentiation. This gene plays a crucial role in neural crest
development, and it can undergo oncogenic activation in vivo and in
vitro by cytogenetic rearrangement. Mutations in this gene are
associated with the disorders multiple endocrine neoplasia, type
IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease,
and medullary thyroid carcinoma. Two transcript variants encoding
different isoforms have been found for this gene. Additional
transcript variants have been described but their biological
validity has not been confirmed.

Specification
Aliases	Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret, Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment, RET, CDHF12, CDHR16, PTC, RET51
Entrez GeneID	5979
Swissprot	P07949
WB Predicted band size	124.3kDa
Host/Isotype	Mouse IgM
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This RET Monoclonal antibody was raised using purified His-tagged recombinant human RET.
Formulation	Mouse monoclonal antibody supplied in crude ascites with 0.05% sodium azide.

Application
WB	1/500-1/16000
IHC	1/100-1/500
ICC	1/10-1/50

	RET (Cat. #P30786) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the RET antibody detected the RET protein (arrow).
	Anti-RET Antibody (Ascites) at 1:1000 dilution + mouse heart lysate Secondary Goat Anti-mouse IgM, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 124319 Da Blocking/Dilution buffer: 5% NFDM/TBST.
	Confocal immunofluorescent analysis of RET Antibody (Ascites)(Cat#P30786) with MDA-MB231 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	RET Monoclonal( Ascites) (Cat. #P30786) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RET Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.