中文 
别名:Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. |
| Specification | |
|---|---|
| Aliases | Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A |
| Entrez GeneID | 6259 |
| Swissprot | P34925 |
| WB Predicted band size | 67.8kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK. |
| Application | |
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
 |
Anti-RYK Antibody at 1:1000 dilution + human lung lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Overlay histogram showing A431 cells stained with P33893(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33893, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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