Mouse Monoclonal Antibody to SP1

包装: 1G|100G|500G|1KG|100KG

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别名:Transcription factor Sp1, SP1, TSFP1
应用:WB,ICC,FCM
反应种属:Human
规格：50μl/100μl

Description
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.

Description

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.

Specification
Aliases	Transcription factor Sp1, SP1, TSFP1
Entrez GeneID	6667
Swissprot	P08047
WB Predicted band size	80.7kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.

Application
WB	1/1000
ICC	1/25
FCM	1/25

	Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibody(Cat. #P32591). P32591 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
	Fluorescent image of Hela cells stained with SP1 Antibody(Cat#P32591). P32591 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
	Flow cytometric analysis of Hela cells using SP1 Antibody(green, Cat#P32591) compared to an isotype control of mouse IgG1(blue). P32591 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.