Mouse Monoclonal Antibody to TGFB2

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别名:Transforming growth factor beta-2, TGF-beta-2, BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF, Polyergin, Latency-associated peptide, LAP, TGFB2
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters’ anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified.

Description

This gene encodes a member of the transforming growth
factor beta (TGFB) family of cytokines, which are multifunctional
peptides that regulate proliferation, differentiation, adhesion,
migration, and other functions in many cell types by transducing
their signal through combinations of transmembrane type I and type
II receptors (TGFBR1 and TGFBR2) and their downstream effectors,
the SMAD proteins. Disruption of the TGFB/SMAD pathway has been
implicated in a variety of human cancers. The encoded protein is
secreted and has suppressive effects of interleukin-2 dependent
T-cell growth. Translocation t(1;7)(q41;p21) between this gene and
HDAC9 is associated with Peters’ anomaly, a congenital defect of
the anterior chamber of the eye. The knockout mice lacking this
gene show perinatal mortality and a wide range of developmental,
including cardiac, defects. Alternatively spliced transcript
variants encoding different isoforms have been identified.

Specification
Aliases	Transforming growth factor beta-2, TGF-beta-2, BSC-1 cell growth inhibitor, Cetermin, Glioblastoma-derived T-cell suppressor factor, G-TSF, Polyergin, Latency-associated peptide, LAP, TGFB2
Entrez GeneID	7042
Swissprot	P61812
WB Predicted band size	47.7kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This TGFB2 monoclonal antibody is generated from mouse immunized with TGFB2 recombinant protein.
Formulation	Purified monoclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Application
WB	1/500-1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	All lanes : Anti- at 1:500-1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	TGFB2/MB10181 antibody (Cat. #P34059) western blot analysis in A549 cell line lysates (35μg/lane).This demonstrates the TGFB2/MB10181 antibody detected the TGFB2/MB10181 protein (arrow).
	Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1(Cat#am1893b). am1893b was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
	TGFB2 Antibody (Cat. #P34059)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TGFB2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	TGFB2 Antibody (Cat. #P34059)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TGFB2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.