中文 
别名:Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. |
| Specification | |
|---|---|
| Aliases | Metalloproteinase inhibitor 2, CSC-21K, Tissue inhibitor of metalloproteinases 2, TIMP-2, TIMP2 |
| Entrez GeneID | 7077 |
| Swissprot | P16035 |
| WB Predicted band size | 24.4kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TIMP2 antibody is generated from a mouse immunized with arecombinant protein of human TIMP2. |
| Application | |
|---|---|
| WB | 1/500-1/2000 |
| IHC | 1/100-1/500 |
| ICC | 1/25 |
| FCM | 1/25 |
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Anti-TIMP2 Antibody at 1:500 dilution + SW480 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Anti-TIMP2 Antibody at 1:2000 dilution + HT-1080 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling TIMP2 with P33380 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue). |
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Overlay histogram showing K562 cells stained with P33380 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33380, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
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Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1(Cat#P33380). P33380 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. |
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P33380 staining TIMP2 in human kidney sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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