中文 
别名:TOP2; TP2A应用:WB,ICC,FCM
反应种属:Human, Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | TOP2; TP2A |
| Entrez GeneID | 7153 |
| Swissprot | P11388 |
| clone | 6C12G12 |
| WB Predicted band size | 174.4kDa |
| Host/Isotype | Mouse IgG2b |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| ICC | 1/100 – 1/500 |
| FCM | 1/200-1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng) |
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Western blot analysis using TOP2A mouse mAb against PC-12 (1), Hela (2), Jurkat (3), and K562 (4) cell lysate. |
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Immunofluorescence analysis of Hela cells using TOP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. |
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Immunofluorescence analysis of Hela cells using TOP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of Hela cells using TOP2A mouse mAb (green) and negative control (red). |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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