Mouse Monoclonal Antibody to VAMP8 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Description

SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

Specification
Aliases	Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8
Entrez GeneID	8673
Swissprot	Q9BV40
WB Predicted band size	11.4kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This VAMP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 2~24 amino acids from the N-terminal region of human VAMP8.

Application
WB	1/1000-1/2000
IHC	1/100-1/500

	Western blot analysis of lysates from Hela, THP-1, A431 cell line (from left to right), using VAMP8 Antibody (N-term)(Cat. #P32781). P32781 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
	Immunohistochemical analysis of paraffin-embedded H. brain section using VAMP8 Antibody (N-term)(Cat#P32781). P32781 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.