别名:PNAS-132;hFL(2)D;KIAA0105应用:WB,IHC,ICC,FCM
反应种属:Human,Monkey
规格:50μl/100μl
| Specification | |
|---|---|
| Aliases | PNAS-132;hFL(2)D;KIAA0105 |
| Entrez GeneID | 9589 |
| Swissprot | Q15007 |
| clone | 6B6B6 |
| WB Predicted band size | 44.2kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Application | |
|---|---|
| WB | 1/500 – 1/2000 |
| IHC | 1/200 – 1/1000 |
| ICC | 1/200 – 1/1000 |
| FCM | 1/200 – 1/400 |
| ELISA | 1/10000 |
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
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Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate. |
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Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503) |
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Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red). |
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining. |
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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