Mouse Monoclonal Antibody to XRCC4

包装: 1G|100G|500G|1KG|100KG

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别名:X ray repair cross complementing protein 4; DNA repair protein XRCC4; DNA double strand break repair
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Description

Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Specification
Aliases	X ray repair cross complementing protein 4; DNA repair protein XRCC4; DNA double strand break repair
Entrez GeneID	7518
Swissprot	Q13426
clone	7C5
WB Predicted band size	Calculated MW: 38 kDa; Observed MW: 38-45 kDa
Host/Isotype	Mouse IgG1
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthetic Peptide of XRCC4
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of XRCC4 in XRCC4 in T47D, 3T3, COS7, C6 and Hela lysates using XRCC4 antibody
	Immunoprecipitation analysis of XRCC4 in Hela lysates using XRCC4 antibody.
	Immunohistochemistry analysis of paraffin-embedded XRCC4 in Human lung cancer using XRCC4 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.