Rabbit Monoclonal Antibody to Acetyl-p53 (Lys370)

包装: 1G|100G|500G|1KG|100KG

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别名:P53; BCC7; LFS1; TRP53
应用:WB,ICC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Description

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.

Specification
Aliases	P53; BCC7; LFS1; TRP53
Entrez GeneID	7157
Swissprot	P04637
WB Predicted band size	Calculated MW: 44 kDa; Observed MW: 53 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic acetyl-peptide corresponding to residues surrounding Lys370 of human p53
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
ICC	1/50-1/200

	Western blot analysis of Acetyl-p53 (Lys370) in Hela, Hela+TSA, 3T3, 3T3+TSA lysates using Acetyl-p53 (Lys370) antibody.
	Western blot analysis of Acetyl-p53 (Lys370) in rat Brain lysates using Acetyl-p53 (Lys370) antibody
	Immunocytochemistry analysis of Acetyl-p53 (Lys370) (green) in 3T3 using Acetyl-p53 (Lys370) antibody,and DAPI(blue).