Rabbit Monoclonal Antibody to AKT

包装: 1G|100G|500G|1KG|100KG

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别名:AKT1
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse,Rat,Hamster
规格：50μl/100μl

Description
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Description

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Specification
Aliases	AKT1
Entrez GeneID	207/208/10000
Swissprot	P31749/P31751/Q9Y243
WB Predicted band size	Calculated MW: 56 kDa; Observed MW: 56 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Hamster
Immunogen	Recombinant protein of human AKT1/2/3
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of AKT in 3T3, mouse lung lysates using AKT antibody.
	Western blot analysis of AKT1/2/3 in Jurkat, rat Brain, C6, CHO-K1, Hela lysates using AKT antibody.
	Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.