Rabbit Monoclonal Antibody to Alkaline Phosphatase

包装: 1G|100G|500G|1KG|100KG

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别名:ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
应用:WB,IHC,ICC,IP
反应种属:Human,Rat
规格：50μl/100μl

Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Specification
Aliases	ALPL; Alkaline phosphatase; tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
Entrez GeneID	249
Swissprot	P05186
WB Predicted band size	Calculated MW: 57 kDa; Observed MW: 75 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	A synthetic peptide of human Alkaline Phosphatase, Tissue Non-Specific
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Alkaline Phosphatase in Hela, C6 lysates using Alkaline Phosphatase antibody.
	Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Alkaline Phosphatase, NonSpecific antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
	Immunocytochemistry analysis of Alkaline Phosphatase, NonSpecific (green) in hela using Alkaline Phosphatase, NonSpecific antibody,and DAPI(blue)