中文 
别名:APG3; APG3L; PC3-96; APG3-LIKE应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl
| Description |
|---|
| E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). Catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. |
| Specification | |
|---|---|
| Aliases | APG3; APG3L; PC3-96; APG3-LIKE |
| Entrez GeneID | 64422 |
| Swissprot | Q9NT62 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ATG3 |
| Formulation | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Application | |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| ICC | 1/50-1/200 |
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Western blot analysis of ATG3 in K562, rat Brain, C6, 3T3, Hela lysates using ATG3 antibody. |
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Immunocytochemistry analysis of ATG3(green) in Hela using ATG3 antibody,and DAPI(blue) |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil using ATG3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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