Rabbit Monoclonal Antibody to CDK9

包装: 1G|100G|500G|1KG|100KG

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别名:TAK; C-2k; CTK1; CDC2L4; PITALRE
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP.

Description

Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP.

Specification
Aliases	TAK; C-2k; CTK1; CDC2L4; PITALRE
Entrez GeneID	1025
Swissprot	P50750
WB Predicted band size	Calculated MW: 43 kDa; Observed MW: 43 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human Cdk9
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Cdk9 in K562, C6, 3T3, Hela lysates using CDK9 antibody.
	Immunocytochemistry analysis of Cdk9(green) in Hela using Cdk9 antibody,and DAPI(blue)
	Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk9 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.