Rabbit Monoclonal Antibody to Cyclin E2

包装: 1G|100G|500G|1KG|100KG

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别名:CCNE2; G1/S-specific cyclin-E2
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers.

Description

The human Cyclin E2 gene encodes a 404 amino acid protein that is most closely related to Cyclin E. Cyclin E2 mRNA levels peaks at the G1 / S transition. Cyclin E2 associates with Cdk2 in a functional kinase complex that is inhibited by both p27 (Kip1) and p21 (Cip1). Cyclin E2 / Cdk2 phosphorylates histone H1 in vitro. G1 cyclin E controls the initiation of DNA synthesis by activating CDK2. Abnormally high levels of cyclin E expression have frequently been observed in human cancers.

Specification
Aliases	CCNE2; G1/S-specific cyclin-E2
Entrez GeneID	9134
Swissprot	O96020
WB Predicted band size	Calculated MW: 47 kDa; Observed MW: 47 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	A synthetic peptide of human Cyclin E2
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Cyclin E2 in Jurkat lysates using Cyclin E2 antibody.
	Immunocytochemistry analysis of Cyclin E2 (green) in MCF-7 using Cyclin E2 antibody,and DAPI(blue)
	Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cyclin E2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.