Rabbit Monoclonal Antibody to Glutaminase

包装: 1G|100G|500G|1KG|100KG

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别名:Glutaminase kidney isoform; GLS; GLS1; KGA; K-glutaminase; GAM; GAC; Glutaminase C; L-glutamine amidohydrolase
应用:WB,ICC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Isoform 1 and isoform 3 are activated by phosphate. Inhibited by BPTES. BPTES binds between subunits and favors dissociation of the tetramer into dimers.

Description

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Isoform 1 and isoform 3 are activated by phosphate. Inhibited by BPTES. BPTES binds between subunits and favors dissociation of the tetramer into dimers.

Specification
Aliases	Glutaminase kidney isoform; GLS; GLS1; KGA; K-glutaminase; GAM; GAC; Glutaminase C; L-glutamine amidohydrolase
Entrez GeneID	2744
Swissprot	O94925
WB Predicted band size	Calculated MW: 73 kDa; Observed MW: 68 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Recombinant protein of human Glutaminase C
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
ICC	1/50-1/200

	Western blot analysis of Glutaminase C in rat brain lysates using Glutaminase C antibody.
	Western blot analysis of Glutaminase in K562, 3T3, Hela lysates using Glutaminase antibody
	Immunocytochemistry analysis of Glutaminase (green) in A549 using Glutaminase antibody,and DAPI(blue).