Rabbit Monoclonal Antibody to Hsp70

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别名:HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A.
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Description

In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Specification
Aliases	HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A.
Entrez GeneID	3303
Swissprot	P0DMV8
WB Predicted band size	Calculated MW: 70 kDa; Observed MW: 70 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human Hsp70
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Hsp70 in K562, rat Brain, C6, 3T3 lysates using Hsp70 antibody.
	Immunocytochemistry analysis of Hsp70(green) in Hela using Hsp70 antibody,and DAPI(blue)
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using Hsp70 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.