Rabbit Monoclonal Antibody to MAPK6

包装: 1G|100G|500G|1KG|100KG

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别名:ERK3; PRKM6; p97MAPK; HsT17250; ERK-3
应用:WB,IHC,IP
反应种属:Human,Mouse
规格：50μl/100μl

Description
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle .

Description

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle .

Specification
Aliases	ERK3; PRKM6; p97MAPK; HsT17250; ERK-3
Entrez GeneID	5597
Swissprot	Q16659
WB Predicted band size	Calculated MW: 83 kDa; Observed MW: 105 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	A synthetic peptide of human MAPK6
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of MAPK6 in 3T3, Hela lysates using MAPK6 antibody.
	Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MAPK6/ERK3 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.