Rabbit Monoclonal Antibody to NELFE

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别名:RD; RDP; RDBP; D6S45; NELF-E
应用:WB,IHC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Specification
Aliases	RD; RDP; RDBP; D6S45; NELF-E
Entrez GeneID	7936
Swissprot	P18615
WB Predicted band size	Calculated MW: 43 kDa; Observed MW: 43 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Recombinant protein of human NELFe
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of NELFe in C6 lysates using NELFE antibody.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NELFe antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.