Rabbit Monoclonal Antibody to Phospho-GSK3 beta (Ser9)

包装: 1G|100G|500G|1KG|100KG

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别名:GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.

Description

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.

Specification
Aliases	GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Entrez GeneID	2932
Swissprot	P49841
WB Predicted band size	Calculated MW: 47 kDa; Observed MW: 47 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK3 beta
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of Phospho-GSK3 beta (Ser9) in K562, C6, 3T3, Hela lysates using Phospho-GSK3 beta (Ser9) antibody.
	Immunocytochemistry analysis of Phospho-GSK3 beta (Ser9)(green) in Hela using Phospho-GSK3 beta (Ser9) antibody,and DAPI(blue)
	Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Phospho-GSK3 beta (Ser9) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.